Turn an EMSA (electrophoretic mobility shift assay) gel image into a binding curve and a dissociation constant. Load a JPEG, PNG or 16-bit TIFF, crop and straighten it, place lanes and the bound and free bands automatically or by hand, enter the protein concentration for each lane and read off Kd with a 95% confidence interval.
Hill, hyperbolic and tight-binding fits; background correction; overlay of titrations; replicate statistics; band intensity on any gel. Everything runs in your browser and nothing is uploaded. Illustrated guide · About